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MyC-CaP
MyC-CaP
規(guī)格:
貨期:
編號(hào):B161410
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 MyC-CaP
商品貨號(hào) B161410
Organism Mus musculus, mouse
Tissue prostate
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease prostate cancer
Age 16 months
Gender male
Strain FVB; Hi-Myc transgenic
Applications Study of prostate cancer progression from androgen dependence to a hormone-refractory or androgen-independent stage
Storage Conditions liquid nitrogen vapor phase
Images Cell Micrograph of MyC-CaP Mouse Prostate Cells
Derivation The MyC-CaP cell line was derived from a genetically engineered mouse prostate cancer removed from an animal that was never exposed to hormone ablation.
Receptor Expression wild-type androgen receptor (AR)
Comments The cells have an amplified androgen receptor gene despite no prior exposure to androgen withdrawal. These cells express wild-type androgen receptor (AR), retain androgen-dependent transgene expression and demonstrate androgen-dependent growth on soft agar and in mice.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Seeding Density 5 x103 to 1 x 104 viable cells/cm2
Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC® 30-2200) or 0.05% Trypsin – 0.02% EDTA for Primary Cells (ATCC® PCS-999-003) solution to remove all traces of serum which contains trypsin inhibitor.
  2. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  4. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.
  5. Discard supernatant. Resuspend the cell pellet in fresh growth medium.
  6. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

Subcultivation ratio: 1:5 to 1:20 is recommended.

Medium renewal: 2 to 3 times a week
Cryopreservation complete growth medium, 90%; DMSO, 10%
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Population Doubling Time 18 hours
Name of Depositor CL Sawyers
Year of Origin 2005
References

Watson PA, et al. Context-dependent hormone-refractory progression revealed through characterization of a novel murine prostate cancer cell line. Cancer Res. 65(24): 11565-11571, 2005. PubMed: 16357166

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