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pNO1523
pNO1523
規(guī)格:
貨期:
編號:B161717
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pNO1523
商品貨號 B161717
Designations pNO1523
Depositors D Dean
Biosafety Level 1
Vector Information
Size (kb): 5.1999998092651370
Vector: pNO1523 (plasmid)
Promoters: Promoter str
Construction: pNO1517
Marker(s):ampR
Construct size (kb): 5.199999809265137
Features: insert detection: rpsL+
marker(s): ampR
promoter: str
replicon: pMB1
Applications
vector permitting positive selection for inserts
Comments
Restriction digests of the clone give the following sizes (kb): SmaI--5.2; EcoRI--5.2.
A vector for positive selection of clones with inserts based on the dominance of the plasmid-coded rpsL+ gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Dean D. A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids. Gene 15: 99-102, 1981. PubMed: 6271634

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