| 產(chǎn)品名稱(chēng) |
pHPT5 |
| 商品貨號(hào) |
B162360 |
| Designations |
pHPT5 |
| Species |
Mus musculus, mouse |
| Depositors |
C Caskey |
| Vector |
Construct size (kb): 5.599999904632568 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.299999952316284 Tissue: neuroblastoma NBR4 cell line Gene product: hypoxanthine phosphoribosyltransferase [Hprt] |
| Insert Size (kb) |
1.300 |
| Media |
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Insert contains the following sites separated by (bp)(approx): (PstI)- 160- AccI- 260- HincII- 115- BstNI- 40- HindIII- 125- HinfI- 55- HhaI- 25- HinfI- 60- HindIII- 100- HaeIII- 195- HinfI- 110- HincII- 55- (PstI). Restriction digests of the clone give the following sizes (kb): PstI--4.4, 1.3. A full-length cDNA clone. There is a high degree of homology within the coding regions between the mouse (ATCC 37424) and the hamster (ATCC 37430) sequences. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Konecki DS, et al. Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis od cDNA recombinants. Nucleic Acids Res. 10: 6763-6775, 1982. PubMed: 6294614
C. Thomas Caskey, personal communication
|
