| 產(chǎn)品名稱 |
514 |
| 商品貨號(hào) |
B163776 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Leukemia |
| Applications |
Animals were immunized with BB2800 leukemia cells which produce both Friend MuLV (FrMuLV) and mink cell focus inducing (MCF) FrMuLV. The antibody neutralizes most MCF and polytropic MuLV, and reacts well with live or fixed infected cells and with Western blots. The antibody efficiently immunoprecipitates gp70, and can be used to distinguish polytropic MuLV from ecotropic, xenotropic and amphotropic MuLV. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against gp70 envelop antigen (ENV) protein of murine leukemia viruses (MuLV) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against gp70 envelop antigen (ENV) protein of murine leukemia viruses (MuLV) |
| Comments |
Animals were immunized with BB2800 leukemia cells which produce both Friend MuLV (FrMuLV) and mink cell focus inducing (MCF) FrMuLV. Spleen cells were fused with NS-1 myeloma cells. The antibody neutralizes most MCF and polytropic MuLV, and reacts well with live or fixed infected cells and with Western blots. The antibody efficiently immunoprecipitates gp70, and can be used to distinguish polytropic MuLV from ecotropic, xenotropic and amphotropic MuLV. |
| Complete Growth Medium |
The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006). To make the complete growth medium, add the following components to the 500 mL of the base medium:
- 10% fetal bovine serum (FBS; ATCC 30-2020)
- 10 µg/mL insulin
- 5.5 µg/mL transferrin
- 5 ng/mL selenium
- 10 ng/mL EGF
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
B Chesebro |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011
|
