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C273
C273
規(guī)格:
貨期:
編號(hào):B164079
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 C273
商品貨號(hào) B164079
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
C273 is was derived from the Sp2/0 hybridoma cell line.
The cells are neomycin resistant and express chimeric heavy and light chain antibody genes encoding the variable region of the mouse monoclonal antibody produced by 2H7 and a human constant region.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
C273 is was derived from the Sp2/0 hybridoma cell line.
The cells are neomycin resistant and express chimeric heavy and light chain antibody genes encoding the variable region of the mouse monoclonal antibody produced by 2H7 and a human constant region.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD20 (chimeric immunoglobulin))
Cellular Products
immunoglobulin; monoclonal antibody; against human CD20 (chimeric immunoglobulin))
Comments
C273 is was derived from the Sp2/0 hybridoma cell line.
It is transfected with plasmids pING2101 and pING2106.
The cells are neomycin resistant and express chimeric heavy and light chain antibody genes encoding the variable region of the mouse monoclonal antibody produced by 2H7 and a human constant region.
Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor Xoma Corporation
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Robinson RR, et al. Chimeric antibody with specificity to human B cell surface antigen. US Patent 5,500,362 dated Mar 19 1996

Clark EA, et al. Role of the Bp35 cell surface polypeptide in human B-cell activation. Proc. Natl. Acad. Sci. USA 82: 1766-1770, 1985. PubMed: 3872456

Profous-Juchelka H, et al. Recombinant and native group B eimeria tenella immunogens useful as coccidiosis vaccines. US Patent 5,824,656 dated Oct 20 1998

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