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FHC
FHC
規(guī)格:
貨期:
編號:B164454
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板 



  
    

      產(chǎn)品名稱
      FHC
    

    

      商品貨號
      B164454
    

      

      Organism
      Homo sapiens, human
    

      

      Tissue
      colon
    

      

      Cell Type
      Epithelial
    

      

      Product Format
      frozen
    

      

      Morphology
      epithelial
    

      

      Culture Properties
      adherent
    

      

      Biosafety Level
      1 




Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    

      

      Disease
      normal
    

      

      Age
      13 weeks gestation
    

      

      Storage Conditions
      liquid nitrogen vapor phase
    

      

      Images
      
    

      

      Comments
      
When using a non-humidified incubator, gassing of cultures with 5% CO2 and plug-seal caps should be used to prevent evaporation of liquid in media and unintentional concentrating of media additives.
Although FHC cells exhibit epithelial morphology, cytoplasmic keratins were not detected.

    

      

      Complete Growth Medium
      
The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006).To make the complete growth medium, add the following components to the base medium:


    
    
  • extra 10 mM HEPES (for a final conc. of 25 mM)
    • 
    
  • 10 ng/ml cholera toxin
    • 
    
  • 0.005 mg/ml insulin
    • 
    
  • 0.005 mg/ml transferrin
    • 
    
  • 100 ng/ml hydrocortisone
    • 
    
  • 20 ng/mL human recombinant EGF (Thermo Fisher PHG0311)
    • 
    
  • Fetal Bovine Serum, 10% final conc (ATCC 30-2020)
    • 






    
        

            The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
            
    
                
  • extra 10 mM HEPES (for a final conc. of 25 mM)
    • 
                
  • 10 ng/ml cholera toxin
    • 
                
  • 0.005 mg/ml insulin
    • 
                
  • 0.005 mg/ml transferrin
    • 
                
  • 100 ng/ml hydrocortisone
    • 
                
  • fetal bovine serum 10%(final conc.
    • 
            

    

      

      Subculturing
      The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
            
    
                
  • extra 10 mM HEPES (for a final conc. of 25 mM)
    • 
                
  • 10 ng/ml cholera toxin
    • 
                
  • 0.005 mg/ml insulin
    • 
                
  • 0.005 mg/ml transferrin
    • 
                
  • 100 ng/ml hydrocortisone
    • 
                
  • fetal bovine serum 10%(final conc.
    • 
            

    

      

      Cryopreservation
      
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.



    
    
  1. Remove and discard culture medium.
    2. 
    
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. 
    
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. 
    
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
    5. 
    
  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 4 to 6 X 103 viable cell/cm2 is recommended. 
    6. 
    
  6. Incubate cultures at 37°C.
    7. 



Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended every 10 to 15 days


Medium Renewal: Every 3 to 4 days.

    

      

      Culture Conditions
      
Freeze Medium: Complete growth medium supplemented with an extra 50% FBS and 10% (v/v) DMSO 


Storage Temperature: Liquid nitrogen vapor phase

    

      

      STR Profile
      
Temperature: 37°C


Atmosphere: Air, 95%; carbon dioxide (CO2), 5%

    

      

      Name of Depositor
      
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12,13
D16S539: 9,11
D5S818: 12,13
D7S820: 8,12
THO1: 6,9.3
TPOX: 8,11
vWA: 16

    

      

      Deposited As
      DP Chopra
    

      

      References
      Homo sapiens
    

    




						
					
					

							

							  
							  
							    

							      梅經(jīng)理
							      17280875617
							      1438578920
							    

							    

							      胡經(jīng)理
							      13345964880
							      2438244627
							    

							    

							      周經(jīng)理
							      17757487661
							      1296385441
							    

							    

							      于經(jīng)理
							      18067160830
							      2088210172
							    

							    

							      沈經(jīng)理
							      19548299266
							      2662369050
							    

							    

							      李經(jīng)理
							      13626845108
							      972239479
							    

							    
							  
							

					

				
				

					
				

			
			
		
		

	
  

	









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