| 產(chǎn)品名稱 |
Vaccinia virus ts mutant |
| 商品貨號(hào) |
B214095 |
| Classification |
Poxviridae, Orthopoxvirus |
| Agent |
Vaccinia virus ts mutant |
| Strain |
IHD-W Dts12 |
| Applications |
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, a viral ATPase required for DNA replication. |
| Biosafety Level |
2 BSL 2Vaccination recommended for laboratory personnel manipulating vaccinia, monkeypox, or cowpox virusesref
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
This conditional lethal mutant, originally designated plaque isolate no. 6741, was isolated from the IHD-W strain of vaccinia by S. Dales. The IHD-W strain was derived from the IHD-J strain. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Not described in the original publication, but presumed to have been selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5 C. This mutant was not assigned to a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, a viral ATPase required for DNA replication. |
| Effect on Host |
Cytopathic effects in cell culture include enlargement and rounding of cells, followed by death and slouging CPE: enlargement, plaques with rounding and eventual sloughing |
| Growth Medium |
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
|
| Recommended Host |
Production Host: BSC-40 (ATCC CRL-2761) Recommended Host: BSC-40 (ATCC CRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2 |
| Growth Conditions |
1-3 days at 31 C with 5% CO2 |
| Name of Depositor |
S. Dales, R. Condit |
| References |
Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807
Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746
Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
|
