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當(dāng)前位置: 首頁 > ATCC代理 > MEF (C57BL/6) [MEF-BL/6-1]
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MEF (C57BL/6) [MEF-BL/6-1]
MEF (C57BL/6) [MEF-BL/6-1]
規(guī)格:
貨期:
編號:B214193
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板 



  
    

      產(chǎn)品名稱
      MEF (C57BL/6) [MEF-BL/6-1]
    

    

      商品貨號
      B214193
    

      

      Organism
      Mus musculus, mouse
    

      

      Tissue
      
Embryo

    

      

      Cell Type
      Fibroblast
    

      

      Product Format
      frozen
    

      

      Morphology
      Fibroblast
    

      

      Culture Properties
      Adherent
    

      

      Biosafety Level
      1 




Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    

      

      Age
      14 days gestation embryo
    

      

      Gender
      male and female mixed
    

      

      Strain
      C57BL/6
    

      

      Applications
      
The cells can be used as a feeder layer to support the growth of embryonic stem (ES) cells and for the maintenance of ES cells in the undifferentiated state. The growth of these cells must be arrested before they can be used  as a feeder layer. ATCC has successfully  treated the cells with mitomycin C for use as a feeder layer. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 6 (P6).

    

      

      Storage Conditions
      liquid nitrogen vapor phase
    

      

      Derivation
      
The cell line was established by ATCC in 2003 from dissociated C57BL/6 mouse embryos.

    

      

      Clinical Data
      
male and female mixed

    

      

      Complete Growth Medium
      The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.  To make the complete growth medium, add the following components to the base medium:  
  • fetal bovine serum to a final concentration of 15%

    • This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
        
    		
    
    
      
    
      Subculturing
      To insure the highest level of viability, be sure to warm media and Trypsin / EDTA to 37ºC before using it on the cells. Cells should be split when they reach confluency. A split base on seed density of 2 X 104 cells/cm2 is recommended.

      
    
    1. Remove and discard culture medium.
      2. 
    
    2. Briefly rinse the cell layer with 1XPBS (SCRR-2201) solution to remove all traces of serum, which contain trypsin inhibitor.
      3. 
    
    3. Add Trypsin-EDTA (0.25% Trypsin-0.53 mM EDTA solution, ATCC# 30-2101) solution to the flask (Table 1) and incubate for 2 minutes. Gently tapping the flask, observe cells under an inverted microscope. Cells usually detach in 2 to 3 minutes.
      4. 
    
    4. Add an equal volume complete of the growth medium (Table 1) and rinse surface of the flask to detach all the cells. Gently pipetting up and down will break cell clumps.  
      5. 
    
    5. Transfer all cells into a centrifuge bottle or tube and centrifuge at 270 x g for 5 minutes.
      6. 
    
    6. Remove and discard the supernatant
      7. 
    
    7. Add 10 mL complete growth medium to the cell pellet and with 10 mL pipette resuspend the cells gently (create a single-cell suspension).
      8. 
    
    8. Add more complete growth medium (Table 1) to the cell suspension as needed to plate cells at approximately 0.8 X 104 cells/cm2.
      9. 
    
    9. Place flasks in the incubator @ 37°C with a 5% CO2 in air atmosphere  
      10. 

    

    

    
    
        
    
            
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    Flask/Plate 
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    		
    
    
      
    
      Cryopreservation
      
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    Growth Area (cm2) 
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    		
    
    
      
    
      Culture Conditions
      
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     1xPBS (mL)
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    		
    
    
      
    
      Name of Depositor
      
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    Trypsin/EDTA (mL) 
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    		
    
    
      
    
      Year of Origin
      
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    Equal vol. Complete Growth Medium (mL) 
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    		
    
    
      
    
      References
      
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    Growth Medium (mL) 
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
     
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    
            
    		
    
    
        

    

						
					
					
    
							
    
							  
							  
							    
    
							      梅經(jīng)理
							      17280875617
							      1438578920
							    
    
							    
    
							      胡經(jīng)理
							      13345964880
							      2438244627
							    
    
							    
    
							      周經(jīng)理
							      17757487661
							      1296385441
							    
    
							    
    
							      于經(jīng)理
							      18067160830
							      2088210172
							    
    
							    
    
							      沈經(jīng)理
							      19548299266
							      2662369050
							    
    
							    
    
							      李經(jīng)理
							      13626845108
							      972239479