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pSGT
pSGT
規(guī)格:
貨期:
編號(hào):B215491
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pSGT
商品貨號(hào) B215491
Designations pSGT
Species Homo sapiens, human
Depositors B Thorens
Vector
Construct size (kb): 5.449999809265137
Insert
DNA: cDNA
Insert lengths(kb): 2.47000002861023
Tissue: hepatocellular carcinoma Hep G2 cell line
Gene product: solute carrier family 2 (facilitated glucose transporter), member 1(glucose transporter 1) [GLUT]
Insert Size (kb) 2.470
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.3, 0.38; BamHI--3.0, 2.45; HindIII--4.65, 0.58; PstI--3.0, 1.65, 0.54, 0.21; ClaI--uncut.
The cDNA insert extends from a synthetic BamHI site introduced at the HaeII site at position -4 to the BamHI site located at position 2469.
References

Mueckler M, et al. Sequence and structure of a human glucose transporter. Science 229: 941-945, 1985. PubMed: 3839598

Mueckler M, Lodish HF. The human glucose transporter can insert posttranslationally into microsomes. Cell 44: 629-637, 1986. PubMed: 3004742

Sarkar HK, et al. Expression of the human erythrocyte glucose transporter in Escherichia coli. Proc. Natl. Acad. Sci. USA 85: 5463-5467, 1988. PubMed: 2840662

Bernard Thorens, personal communication

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