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PKCzeta(FC23)
PKCzeta(FC23)
規(guī)格:
貨期:
編號:B220784
品牌:Mingzhoubio

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DNA
RNA

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甘油
平板


產品名稱 PKCzeta(FC23)
商品貨號 B220784
Designations PKCzeta(FC23)
GenBank Number

L14283

Species Homo sapiens, human
Vector
Construct size (kb): 5.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: SpeI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCZ
Gene name: protein kinase C, zeta
Contains complete coding sequence?: Y
Tissue: brain, frontal cortex
Type of DNA: cDNA
Insert 5' end: SpeI
Insert 3' end: EcoRI
Insert size (kb): 2.0
Cross references: DNA Seq. Acc.: L14283
Insert lengths(kb): 2.0
Tissue: brain, frontal cortex
Gene product: protein kinase C, zeta [PRKCZ]
Insert Size (kb) 2.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--3.0, 2.1; BglII--5.0; PstI--4.8, 0.25; PvuII--2.5, 1.9, 0.7; SacI--4.9, 0.2.
Contains the complete coding sequence only (nt 1-1779).
References

Barbee JL, et al. The cDNA sequence encoding human protein kinase C-zeta. Gene 132: 305-306, 1993. PubMed: 8224878

David J Burns, personal communication

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