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Distribution host: Escherichia coli DH5alpha

Size (kb): 7.5999999046325680

DESCRIPTION OF VECTOR:
Intact vector size: 7.600
Type of vector: plasmid
Cloning sites: XbaI NotI SalI SphI HindIII
Polylinker sites: EcoRI BamHI XbaI SalI PstI SphI HindIII
Construction: pUC19, pC4AUG beta-gal, pL1, pXT1
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: HSV TK
replicon: pMB1
terminator: SV40 late

Vector: pTKbeta (plasmid)

Promoters: Promoter HSV TK

Construction: pUC19, pC4AUG beta-gal, pL1, pXT1

Marker(s):ampR

Construct size (kb): 7.599999904632568

Features: insert detection: lacZ'
marker(s): ampR
promoter: HSV TK
replicon: pMB1
terminator: SV40 late

contains easily purifiable cassette(s) for construction

reporter construct

vector for other uses

vector permitting visual detection of activity in colonies

Restriction digest of the clone give the following sizes (kb): EcoRI--7.2, 0.9; HindIII--7.6; EcoRI/HindIII--4.5, 2.7, 0.9; NotI--4.4, 3.2; BamHI--3.8 (doublet).

Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining.

Designed to express low levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors.

The lacZ gene may be excised by NotI and replaced with alternative sequences.

The order of the major features in this plasmid is: pUC19 - EcoRI - HSV thymidine kinase promoter - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19.

Temperature: 37.0°C