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Human enterovirus 71
Human enterovirus 71
規(guī)格:
貨期:
編號(hào):B221664
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Human enterovirus 71
商品貨號(hào) B221664
Classification Picornaviridae, Enterovirus
Deposited As Enterovirus 71
Agent Human enterovirus 71
Strain H
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Effect on Host CPE, refractile rounding and sloughing
Recommended Host
LLC-MK2 (ATCC® CCL-7.1™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.001). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 2 to 6 days

Name of Depositor ZM Zheng, National Cancer Institute
Source Vesicular fluid from an adult female with hand, foot, and mouth disease, Wuhan, China
References

Zheng ZM, et al. First isolation of enterovirus type 71 from vesicle fluid of an adult patient with hand-foot-mouth disease in China. Virologica Sinica. 4: 375-382, 1989.

Zheng ZM, et al. Comparison of enterovirus 71 (E71) isolated from a patient with hand-foot-and-mouth disease in China to prototype E71 BrCr strain by polymerase chain reaction using a unique primer pair. Clin. Diagn. Virol. 1: 137-139, 1993. PubMed: 15566727

Zheng ZM, et al. Enterovirus 71 isolated from China is serologically similar to the prototype E71 BrCr strain but differs in the 5'-noncoding region. J. Med. Virol. 47: 161-167, 1995. PubMed: 8830120

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