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gp91-phox-cDNA [UTR-01292, WI-7096]
gp91-phox-cDNA [UTR-01292, WI-7096]
規(guī)格:
貨期:
編號:B223967
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 gp91-phox-cDNA [UTR-01292, WI-7096]
商品貨號 B223967
Designations gp91-phox-cDNA [UTR-01292, WI-7096]
GenBank Number

X04011

Species Homo sapiens, human
Vector
Construct size (kb): 4.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.600000023841858
Tissue: HL-60 cell line (promyelocytic leukemia)
Gene product: cytochrome b-245, beta polypeptide (chronic granulomatous disease)(cytochrome b-245, beta polypeptide) [CGD]
Insert Size (kb) 1.600
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.6; XhoI--4.6; PstI/XhoI--3.0, 1.6; EcoRI--4.6; PvuII--2.5, 2.1.
The insert contains the EcoRI site at nt 1570 of the published sequence.
Extends from the PstI site at nt 186 of the published sequence (5' untranslated region) through the complete coding sequence into the 3' untranslated region to the SacI site at about nt 1950.
The polypeptide is encoded from nt 13 to 1728 of the final published sequence.
Oriented such that transcription from the T7 promoter gives the sense strand.
References

Orkin SH. Molecular genetics of chronic granulomatous disease. Annu. Rev. Immunol. 7: 277-307, 1989. PubMed: 2523713

Royer-Pokora B, et al. Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location. Nature 322: 32-38, 1986. PubMed: 2425263

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