| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--22.3, 17.5, 3.2, 1.0, 0.6; EcoRI/HindIII--21.0, 14.0, 4.7, 2.2, 1.65, 1.0, 0.9, 0.6. Verified to contain the correct insert sequence using the following oligonucleotides (5'-3'): AACCCTATACATGTAAACAGTGTGGG and AAAGCCTTTCCCACATATCTTACAT. The predicted product size is (bp): 118. The observed product size is (bp): 118. |
