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Construct size (kb): 7.900000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GRL
Genomic copy number: unique
Gene name: glucocorticoid receptor
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q31-q32
Tissue: leukocyte
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 3.5
Cross references: DNA Seq. Acc.: M73816
Nucleotides 1-1628 of the insert correspond to
nucleotides 1-1628 of M73816 with 100% identity.

Insert lengths(kb): 3.5

Tissue: leukocyte

Gene product: glucocorticoid receptor [GRL]

Restriction digests of the clone give the following sizes (kb): BglI--3.3, 2.4, 2.0, 0.26; EcoRI--4.4, 3.4; HindIII--4.8, 1.1, 0.68, 0.60, 0.50, 0.30; PvuII--3.9, 2.4, 1.8; SalI--6.5, 1.6.

The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.93; BglII--0.64, 1.20; HindIII--0.14, 0.77, 1.34; PvuII--0.16; SalI--0.81.

Contains part of intron 1, exon 2 and part of intron 2.

Subcloned from lambdaGHGR-5. ATCC 80008/9 and ATCC 80010/11 are contiguous in the genome.