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pUMA23

規(guī)格:

貨期:

編號:B243063

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購物車

產(chǎn)品名稱	pUMA23
商品貨號	B243063
Designations	pUMA23
Depositors	UA Heinlein
Biosafety Level	1
Vector Information	Size (kb): 3.0000000000000000 Vector: pUMA23 (plasmid) Promoters: Promoter for in vitro transcription SP6 Construction: pSP64 Marker(s):ampR Construct size (kb): 3.0 Features: initiation codon: ATG marker(s): ampR promoter for in vitro transcription: SP6 replicon: pMB1 MCS: EcoRI...NotI restriction site: NcoI stop codons: 3
Applications	construct to generate mRNA that can be expressed in Xenopus oocytes vector for constructing cDNA libraries vector permitting RNA synthesis in vitro
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--3.0; PstI--3.0; XbaI--3.0. Cloning vector useful for in vitro transcription of cloned inserts. In vitro transcribed sequences will all contain the authentic 5'-UTR of X. laevis beta globin on the 5' end.
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	Dommers S, Heinlein UA. Recombinant polypeptides transplanted from pUMA vector-derived cRNAs are translocated through microsomal membranes and exported out of frog oocytes. Biochem. Biophys. Res. Commun. 204: 1346-1351, 1994. PubMed: 7980614
Shipped	freeze-dried

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