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Adult Melanocyte Growth Kit
Adult Melanocyte Growth Kit
規(guī)格:
貨期:
編號:B244961
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板 


      
  
    
      
      產(chǎn)品名稱
      Adult Melanocyte Growth Kit
    
      
    
      
      商品貨號
      B244961
    
      
      
      
      Biosafety Level
      1 


      

      Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
      		
    
      
      
      
      Product Format
      frozen 1 Kit
    
      
      
      
      Storage Conditions
      -20°C (or -70°C for long-term storage)
    
      
      
      
      Comments
      The Adult Melanocyte Growth Kit contains components that when added to Dermal Cell Basal Medium (ATCC PCS-200-030) create a complete low-serum culture environment for adult melanocytes derived from normal human epidermis (e.g., Primary Epidermal Melanocytes, Normal, Human Adult, ATCC PCS-200-013). The complete low serum medium is formulated to support robust adult melanocyte growth in the absence of cholera toxin and phorbol 12-myristate 13-acetate (PMA).  No feeder layers, extracellular matrix proteins or other substrares are required.
      

      
The final concentration for each component in complete adult melainocyte growth medium is as follows:
      

        
    
      • rh Insulin: 5 µg/mL
        • 
    
      • Ascorbic Acid: 50 µg/mL
        • 
    
      • L-Glutamine: 6 mM
        • 
    
      • Epinephrine: 1.0 µM
        • 
    
      • Calcium chloride: 1.5 mM
        • 
    
      • Peptide Growth Factor: 0.2%, Proprietary formulation
        • 
    
      • M8 Supplement: 1%, Proprietary formulation
        • 

      		
    
      
      
      
      Complete Growth Medium
      
        
    
      1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
        2. 
    
      2. Thaw the components of the growth kit just prior to adding them to the basal medium.
        3. 
    
      3. Obtain one bottle of Dermal Cell Basal Medium (485 mL) from cold storage. 
        4. 
    
      4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
        5. 
    
      5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer. 
        6. 
    
        Table 1. Adult Melanocyte Growth Kit Components  
        
    
        
    
        
        
            
        
                 
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        Component
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
      
        
      Volume
      
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        Volume
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
      
        
      Sterility Tests
      
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        Final Concentration
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
      
        
      Functional Tests
      
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        rh Insulin
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
      
        
      C of A
      
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        0.5 mL
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
            

        

						
					
					
        
							
        
							  
							  
							    
        
							      梅經(jīng)理
							      17280875617
							      1438578920
							    
        
							    
        
							      胡經(jīng)理
							      13345964880
							      2438244627
							    
        
							    
        
							      周經(jīng)理
							      17757487661
							      1296385441
							    
        
							    
        
							      于經(jīng)理
							      18067160830
							      2088210172
							    
        
							    
        
							      沈經(jīng)理
							      19548299266
							      2662369050
							    
        
							    
        
							      李經(jīng)理
							      13626845108
							      972239479